Yes, I solved with the Support ... I opened a case
It was a pemission problem ... I make a copy / paste of our activity (maybe it can help you):
You can find below a summary of the troubleshooting we performed during our call:
We started by reproducing the problem of attempting to create a new application in Service Studio and we immediately obtained the HTTP 401 Unauthorized error;
This was tested from outside the server machine and also from inside the server connected through "localhost";
There was no error logged in the Error logs of Service Center nor any call in the Integration Logs;
From the error in Service Center, we could see that the error was being triggered when calling and exposed REST service from a System Component called Template Manager;
This web service uses Basic Authentication and the authentication token is sent on the headers of the request;
By enabling the IIS feature Failed Request Tracing, we were able to validate that the request from Service Studio did contain the Basic Authentication header, but the error was happening nonetheless;
After analyzing the IIS Authentication on the Template Manager site, we verified that this site had the Basic Authentication enabled. After disabling it, we were able to create new applications;
It seems that the Template Manager inherited the Authentication configurations from the Default Web Site because it is a new module. Modules that already existed remained with the current configurations.
You can also revert the changes we performed during the troubleshooting:
Change the Integration log level of the Template Manager exposed REST service TemplateServices to Default to avoid excessive logging;
Disable the IIS Failed Request Tracing;
Since the problem is resolved, we'll be marking the ticket as solved.